WebBulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental … WebSep 5, 2024 · Bulked segregant analysis (BSA) is a rapid, cost-effective method for mapping mutations and quantitative trait loci (QTLs) in animals and plants based …
Bulked sample analysis in genetics, genomics and crop …
WebNov 1, 2024 · Here, we describe a bulked-segregant analysis sequencing (BSA-Seq) protocol for cloning mutant genes in maize. Our simple strategy can be used to quickly identify a mapping interval and candidate single nucleotide polymorphisms (SNPs) from whole genome sequencing of pooled F2 individuals. WebNov 3, 2011 · In this study we describe an analytical framework that allows investigators to marry a QTL mapping approach called “bulk segregant analysis” (BSA) with high-throughput genome sequencing methodologies in order to map traits quickly, efficiently, and in a relatively inexpensive manner. brentford fc shirt
QTL‐seq: rapid mapping of quantitative trait loci in rice by whole ...
WebAug 21, 2024 · The authors developed a bulked segregant analysis (BSA) method driven by deep learning, DeepBSA, for quantitative trait loci mapping and functional gene … WebBulked segregant analysis ( BSA) is a technique used to identify genetic markers associated with a mutant phenotype. This allows geneticists to discover genes … WebDec 12, 2024 · The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes. Here, we report an improved WGS-BSA method termed as M2-seq to expedite the mapping candidate mutant loci by studying just M-2 generation. It is an efficient mutant gene … countertop gardening systems