Thy1-gcamp6 transgenic mice

Author: mwuj

August undefined, 2024

WebbThe first transgenic mouse expressing GCaMP1 was reported in 2004. However, at 37 ˚C ... GCaMP6, and jGCaMP7, have been developed to progressively improve the signal, sensitivity, and dynamic range of Ca 2 ... researchers have used GCaMP to observe neuronal activity in mice by expressing it under control of the Thy1 promoter, ... WebbVariants of GCaMP fluoresce in response to calcium binding and serve as an indicator of neuronal activation. These include Thy1-promoter driven GCaMP6 transgenic lines, Tet-dependent GCaMP6f or GCaMP6s transgenic lines and Cre-dependent GCaMP3, GCaMP5, GCaMP6f or GCaMP6s mouse lines.

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WebbNew generations of Thy1-XFP mice are now being generated at a high rate, and these have allowed previously difficult experiments to become feasible. Moreover, with easy access to core facilities or commercial providers of pronuclear injections, generating simple Thy1 transgenic mice is now a possibility even for small laboratories. WebbFurther, transgenic mice are now commercially available that express GCaMPs in a Cre recombinase dependent fashion. ... Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. PLoS ONE 9:e108697. doi: 10.1371/journal.pone.0108697. PubMed Abstract Full Text CrossRef Full Text Google Scholar. Datta, S. R., and … ofmem sir reports

GCaMP - Wikipedia

Webb24 sep. 2014 · We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1... Webb7 maj 2024 · In a separate set of experiments using intact cranial windows of Thy1-GCaMP6 transgenic mice to avoid the possible trace left by viral injection, we have set out to identify the possible cell loss ... WebbThese GCaMP reporter mice can be bred to distinct Cre recombinase driver mice to direct expression of this sensor in unique populations of cells. Concerning the central nervous … myflexwork

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Imaging Neural Activity Using Thy1-GCaMP Transgenic …

Webb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy. This method enables recordings and quantifications of neural activity in bilateral mouse brain regions … Webb20 jan. 2024 · Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously described (61). As no significant differences were observed between male and female mice,... myflfamilies dcf trainingWebb7 dec. 2012 · Chen et al. report transgenic lines expressing either GCaMP2.2c or GCaMP3 under the control of the Thy1 promoter. These mice showed GCaMP fluorescence in … my flexti.com

"Webb20 aug. 2024 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. Dana H, Chen TW, Hu A, Shields BC, Guo C, Looger LL, Kim DS, Svoboda K. PLoS One, (9):e108697 2014 MED: 25250714 Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. Dombeck DA, Harvey CD, Tian L ... " - Thy1-gcamp6 transgenic mice

Thy1-gcamp6 transgenic mice

Imaging neural activity using Thy1-GCaMP transgenic mice

Webb23 sep. 2024 · Adult (P65-P70) transgenic mice (C57BL/6J-Tg (Thy1-GCaMP6f) GP5.1Dkim/J; The Jackson Laboratory) expressing GCaMP6f under the Thy1 promoter were used. All animals were housed in a temperature-controlled (24 °C) colony room and maintained in a 12-h/12-h light/dark cycle (lights on at 7:00 a.m.). Food and water were … WebbGCaMP6 functional connectivity maps of all 7 individual mice in the imaging cohort, filtered over the delta (0.4–4.0Hz) frequency band. The same canonical network seeds used in Figs Figs44and and55were applied here. Data shown are acquired in the awake state. (TIF) pone.0185759.s002.tif(2.8M) GUID: C405C9D5-751C-4285-B16C-4C0455F11153

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Webb24 sep. 2014 · Thy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23] .We included the WPRE (Woodchuck hepatitis virus post-transcriptional … WebbGCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the …

Webb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of … Webb1 okt. 2012 · DOI: 10.1016/j.neuron.2012.07.011 Corpus ID: 17493469; Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice @article{Chen2012ImagingNA, title={Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice}, author={Qian Chen and Joseph Cichon and Wenting Wang and Li Qiu and Seok-Jin R. Lee and Nolan R. Campbell and …

WebbHere we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. Webb16 feb. 2024 · Thy1-GCaMP6s transgenic mice have been developed by transgenic meth- ods and express GCaMP6s under the Thy1promoter, in which projection neurons in the cerebral cortex and hippocampus...

WebbFurthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice. View on publisher site Alert me about new mentions. Twitter Demographics. The data shown below were collected from the profiles of 2 tweeters who shared this research output.

Webb18 okt. 2012 · These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting … my flex won\u0027t syncWebb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of … ofmem section 21Webb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of GCaMP2.2c and six founder lines of GCaMP3. Our previous studies have shown that the Thy1 promoter predominantly drives transgene expression in projection neurons in the CNS. my flex won\\u0027t syncWebb23 juli 2024 · For example, Thy1-GCaMP6 mice show stable expression of GCaMP6 in multiple cortical regions across months without apparent toxicity, but only after the third postnatal week (Dana et al., 2014). Similarly, the Ai38 mouse line expresses GCaMP3 when treated with tamoxifen at P7, but showed very low expression at 4 weeks of age … ofmem skill sheetsWebb22 juni 2024 · Expression of GCaMP6 can be obtained using transgenic mice where the gene could be inserted under the Thy1 promoter (Thy1-GCaMP6s, 16), under the control of cre-recombinase and the … ofmem tg-02-2019Webb12 apr. 2024 · Venglustat in Thy1-aSyn Mice. Thy1-aSyn (line 61) transgenic mice overexpressing human WT α-synuclein under the Thy-1 promoter were maintained on a mixed C57BL/6-DBA/2 background as described previously. 18, 20 Polymerase chain reaction amplification analysis was performed on ear tissue samples collected both at … myfl family accessWebb24 sep. 2014 · We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three … ofm ems